Detection of Bacteroides fragilis and Bacteroides melaninogenicus by direct immunofluorescence.
نویسندگان
چکیده
منابع مشابه
Rapid detection and identification of Bacteroides fragilis and Bacteroides melaninogenicus by immunofluorescence.
Bacteroides fragilis group and Bacteroides melaninogenicus group fluorescent-antibody kits were evaluated with 188 clinical specimens and 116 fresh aerobic and anaerobic bacterial isolates. Fluorescent-antibody and culture results corresponded in 88% of clinical specimens of the B. fragilis group and 94% of clinical specimens of the B. melaninogenicus group. There was greater than or equal to 9...
متن کاملCharacterization of bacteroides melaninogenicus.
Fifty-eight human isolates of Bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light fluorescence and by forty biochemical and other tests, including end-product analysis by gas-liquid chromatography. In a number of instances, tests were repeated several times and the results were reproducib...
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Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
متن کاملLipids of Bacteroides melaninogenicus.
The lipids of Bacteroides melaninogenicus were readily extractable with chloroform-methanol. Three per cent of the fatty acids were not extractable. The neutral lipids contained 4% of the extractable fatty acids, the stench characteristic of these organisms, and 0.5 mumole of vitamin K(2) isoprenologues K(2)-35, K(2)-40, and K(2)-45 per g (dry weight). This is one-fifth to one-tenth of the vita...
متن کاملNotes on bacteroides melaninogenicus.
element is still in doubt. Efforts are being directed toward the interpretation of the structures which can be visualized in bacteria (DeLamater, Ann. Rev. Microbiol, 8, 23, 1954). The description and (lemonstration of the nuclear configurations found in normal vegetative cells of S. typhosa form the basis for comparison with cells treated with colchicine described by Minsavage and DeLamater (J...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1980
ISSN: 0021-9746
DOI: 10.1136/jcp.33.12.1189